Mammalian gene expression vectors
In the biomedical field, the use of conventional transfection to transfer plasmid vectors into mammalian cells is the most widely used method.Although many more advanced gene introduction vector systems (such as lentiviral vectors, adenovirus vectors, AAV vectors and piggyBac) have been developed in recent years, conventional plasmid gene expression vectors are still used in most laboratories, mainly due to their technical simplicity and efficient transfection rate in various cell types.The key feature of conventional plasmid vector transfection is transient, and very inefficient integration in the host genome (usually less than 1%).
For more information on Mammalian gene expression vectors, please refer to the following literature.
For more information on Mammalian gene expression vectors, please refer to the following literature.
| Reference Documentation | Theme |
| Mol Biotechnol. 16:151 (2000) | Overview of vector design for mammalian gene expression |
| EMBO J. 12:2539 (1993) | Transcription blocker prevent transcriptional interference |
Previous：Adeno-associated virus vector
Next：Chimeric vectors
